Jump to main content
Jump to site search

Issue 7, 2018
Previous Article Next Article

Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes

Author affiliations

Abstract

Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China. To investigate the active components responsible for its health benefits, a novel heteropolysaccharide GP70-3 was purified by water extraction, ethanol precipitation and column chromatography. Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-D-Galp, 1→5)-linked α-L-Araf, 1→6)-linked β-D-Galp and 1→3)-linked β-D-Galp, branched with 1→2,3,5)-linked α-L-Araf, 1→3)-linked α-L-Araf, 1→3)-linked α-L-Rhap, 1→3)-linked β-D-GlcpA, 1→3)-linked β-D-GalpA and terminated with →1)-linked β-D-Galp. Advanced structure studies showed GP70-3 consisted of irregular flakes with rounded-spherical pores. Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM). Therefore, consumption of guava polysaccharides may be beneficial as an α-glucosidase inhibitor for reducing the postprandial blood glucose level and treating type II diabetes.

Graphical abstract: Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes

Back to tab navigation

Article information


Submitted
25 Apr 2018
Accepted
26 Jun 2018
First published
28 Jun 2018

Food Funct., 2018,9, 3997-4007
Article type
Paper

Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes

Y. Jiao, D. Hua, D. Huang, Q. Zhang and C. Yan, Food Funct., 2018, 9, 3997
DOI: 10.1039/C8FO00790J

Social activity

Search articles by author

Spotlight

Advertisements