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Issue 7, 2018
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Structural characterization of a novel acidic polysaccharide from Rosa roxburghii Tratt fruit and its α-glucosidase inhibitory activity

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Abstract

A novel acidic polysaccharide (RTFP-3) was isolated and purified from Rosa roxburghii Tratt fruits. Structural analyses revealed that RTFP-3 is a homogeneous polysaccharide with a molecular weight of 67.2 kDa and mainly consists of arabinose (37.20%), galactose (34.40%), glucose (10.02%) and fucose (18.30%). The structural chain of RTFP-3 was proven to be composed of →6)-α-D-Galp-(1→, →4)-α-D-GalpA-(1→, →5)-α-L-Araf-(1→, →4)-β-D-Glcp-(1→, β-D-Glcp-(1→ and →3,4)-β-L-Fucp-(1→ residues by periodate oxidation–Smith degradation, methylation, and 1D- and 2D-NMR analyses. Bioassay results showed that RTFP-3 exerted favorable mixed type inhibitory activities against α-glucosidase, and its half inhibitory concentration (IC50) value was determined as 4.15 mg mL−1. RTFP-3 showed static type quenching of the intrinsic fluorescence of α-glucosidase, during which the binding process induced the conformational change of α-glucosidase. These results suggest that RTFP-3 has the potential to be a novel α-glucosidase inhibitory agent for use in functional foods.

Graphical abstract: Structural characterization of a novel acidic polysaccharide from Rosa roxburghii Tratt fruit and its α-glucosidase inhibitory activity

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Supplementary files

Article information


Submitted
23 Mar 2018
Accepted
11 Jun 2018
First published
15 Jun 2018

Food Funct., 2018,9, 3974-3985
Article type
Paper

Structural characterization of a novel acidic polysaccharide from Rosa roxburghii Tratt fruit and its α-glucosidase inhibitory activity

L. Wang, C. Chen, B. Zhang, Q. Huang, X. Fu and C. Li, Food Funct., 2018, 9, 3974
DOI: 10.1039/C8FO00561C

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