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Issue 8, 2016
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Quantification of 4-hydroxy-2-nonenal-protein adducts in the in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

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Abstract

Hydroxyalkenals are lipid oxidation end-products resulting from the oxidation of polyunsaturated fatty acids (PUFA). This study aimed at quantifying the production of 4-hydroxy-2-nonenal-protein adducts (HNE-P) via Michael addition from n-6 PUFA oxidation in the gastric digesta of mini-pigs after the consumption of meat-based meals with different plant antioxidant contents. Using the accuracy profile procedure, we validated an extraction protocol for the quantification of HNE-P by GC-MS/MS in gastric contents. The formation of HNE-P in the gastric compartment was observed for the first time, with concentrations ranging from less than 0.52 to 1.33 nmol HNE-P per 500 mg digesta. Nevertheless, most gastric HNE-P levels were below the limit of quantification of 0.52 nmol HNE-P per 500 mg digesta. In this animal study, the protective effect of plant antioxidant sources on HNE-P formation was not evidenced contrasting with the results using TBARS as markers.

Graphical abstract: Quantification of 4-hydroxy-2-nonenal-protein adducts in the in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

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Article information


Submitted
22 Mar 2016
Accepted
16 Jun 2016
First published
01 Jul 2016

Food Funct., 2016,7, 3497-3504
Article type
Paper

Quantification of 4-hydroxy-2-nonenal-protein adducts in the in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

M. Delosière, V. Santé-Lhoutellier, C. Chantelauze, D. Durand, A. Thomas, C. Joly, E. Pujos-Guillot, D. Rémond, B. Comte, C. Gladine, A. Guy, T. Durand, M. Laurentie and C. Dufour, Food Funct., 2016, 7, 3497
DOI: 10.1039/C6FO00413J

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