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Issue 8, 2014
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N-3 PUFA increase bioavailability and function of endothelial progenitor cells

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Background and Aims: Recent data suggest that n-3 PUFA exert beneficial effects on endothelial progenitor cell (EPC) biology. We sought to investigate whether these effects might be mediated by enhanced EPC in vitro function and/or in vivo bioavailability. Methods and Results: CACs and late-outgrowth EPCs were isolated from peripheral blood mononuclear cells obtained from 12 donor buffy-coats. The effect of n-3 PUFA (EPA : DHA = 0.9 : 1.5; 9 μM EPA plus 15 μM DHA) was tested on CAC/EPC viability, function (tube-formation) and pro-inflammatory molecule expression. Circulating EPC (cells positive for CD34, CD133 and kinase insert domain receptor – KDR cell-surface antigens by flow cytometry) number was evaluated in 20 healthy subjects (10 F/10 M, 32 ± 5 years), randomized to receive 4 mackerel or sardine portions per week for 6 weeks followed by a 6 week free-diet period. N-3 PUFA improved CAC and late-outgrowth EPC viability (p < 0.05) and the capacity to form tube-like structures in CACs (+38%; p < 0.05) and late-outgrowth EPCs (+15%; p < 0.05). ICAM-1 expression was reduced in both CACs (p < 0.05) and late-outgrowth EPCs (p < 0.05) and VCAM-1 in late-outgrowth EPCs (p < 0.005). N-3 PUFA significantly decreased TNF-α and MCP-1 expression in CACs and IL-8, TNF-α and MCP-1 in late-outgrowth EPCs (p < 0.05). Circulating EPC number significantly improved after 6 weeks of a fish-enriched diet (p < 0.01) and returned to baseline levels after a 6 week free-diet period (p < 0.01). Plasma EPA levels were independently and positively associated with EPC levels (p < 0.005). Conclusion: Our findings support the case of a beneficiary role played by n-3 PUFA in EPC function and bioavailability.

Graphical abstract: N-3 PUFA increase bioavailability and function of endothelial progenitor cells

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Publication details

The article was received on 29 Nov 2013, accepted on 15 May 2014 and first published on 15 May 2014

Article type: Paper
DOI: 10.1039/C3FO60641D
Food Funct., 2014,5, 1881-1890

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    N-3 PUFA increase bioavailability and function of endothelial progenitor cells

    V. Spigoni, C. Lombardi, M. Cito, A. Picconi, V. Ridolfi, R. Andreoli, N. Anelli, L. Gnudi, M. Goldoni, I. Zavaroni, R. Raddino and A. D. Cas, Food Funct., 2014, 5, 1881
    DOI: 10.1039/C3FO60641D

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