Jump to main content
Jump to site search

Issue 4, 2012
Previous Article Next Article

Genotoxic potential of methyleugenol and selected methyleugenol metabolites in cultured Chinese hamster V79 cells

Author affiliations

Abstract

Methyleugenol is a substituted alkenylbenzene classified by the European Union's Scientific Committee on Food as a genotoxic carcinogen. We addressed cytotoxicity, genotoxicity and mutagenicity caused by methyleugenol and selected oxidative methyleugenol metabolites in Chinese hamster lung fibroblasts V79 cells. Cytotoxicity was measured by two cell proliferation assays, water soluble tetrazolium salt (WST) 1 and sulforhodamine B (SRB) assays. Genotoxicity was determined by using single cell gel electrophoreses (comet assay) and the in vitro micronuclei test, while mutagenicity was investigated with the hypoxanthinephosphoribosyl transferase (hprt) assay. Methyleugenol and 1′-hydroxymethyleugenol showed no or marginal cytotoxic effects, but caused DNA strand breaks at concentrations ≥10 μM. The metabolites methyleugenol-2′,3′-epoxide and 3′-oxomethylisoeugenol exhibited growth inhibitory properties with IC50-values of 70–90 μM after 48 h or 72 h of incubation. These metabolites significantly enhanced cytotoxicity and DNA damage after 1 h of incubation. Overall, no increase in formamidopyrimidine DNA glycosylase sensitive sites were detected with the comet assay. The metabolites 1′-hydroxymethyleugenol and methyleugenol-2′,3′-epoxide exceeded the DNA strand breaking properties of the parent compound methyleugenol. However, only 3′-oxomethylisoeugenol and methyleugenol-2′,3′-epoxide induced the formation of micronucleated cells in comparison to the negative control. These compounds were found to be not or rather weakly mutagenic at the hprt locus. In summary, phase I metabolites exceeded the cytotoxic and genotoxic properties of the parent compound methyleugenol.

Graphical abstract: Genotoxic potential of methyleugenol and selected methyleugenol metabolites in cultured Chinese hamster V79 cells

Back to tab navigation

Article information


Submitted
10 Oct 2011
Accepted
28 Dec 2011
First published
03 Feb 2012

Food Funct., 2012,3, 428-436
Article type
Paper

Genotoxic potential of methyleugenol and selected methyleugenol metabolites in cultured Chinese hamster V79 cells

I. A. Maria Groh, A. T. Cartus, S. Vallicotti, J. Kajzar, K. Merz, D. Schrenk and M. Esselen, Food Funct., 2012, 3, 428
DOI: 10.1039/C2FO10221H

Social activity

Search articles by author

Spotlight

Advertisements