Jump to main content
Jump to site search

Issue 8, 2012
Previous Article Next Article

Protective role of Brassica olerecea and Eugenia jambolana extracts against H2O2 induced cytotoxicity in H9C2 cells

Author affiliations

Abstract

This study assesses the efficacy of anthocyanin rich Brassica olerecea leaves (ARCE) and flavonoid rich Eugenia jambolana seed (EJSE) extracts as possible cardioprotective agents against hydrogen peroxide (H2O2) induced cytotoxicity in H9C2 cells. Presence of ARCE or EJSE resulted in a superior cell viability and cell integrity as revealed by cell viability and lactate dehydrogenase release assays and acridine orange and ethidium bromide staining of control and H2O2 treated H9C2 cells. These extracts were also able to reduce the impact of H2O2 induced lipid peroxidation and depletion of intracellular glutathione. Also, there was an increase in mitochondrial membrane potential and reduced generation of intracellular reactive oxygen species following ARCE or EJSE treatments. These results suggest that ARCE and EJSE are capable of cardioprotective activity due to the high number of anthocyanins and flavonoids in them that are instrumental in lowering intracellular oxidative stress, preventing depletion of cellular antioxidants and improving cell viability.

Graphical abstract: Protective role of Brassica olerecea and Eugenia jambolana extracts against H2O2 induced cytotoxicity in H9C2 cells

Back to tab navigation

Article information


Submitted
01 Jan 2012
Accepted
18 Apr 2012
First published
17 May 2012

Food Funct., 2012,3, 837-843
Article type
Paper

Protective role of Brassica olerecea and Eugenia jambolana extracts against H2O2 induced cytotoxicity in H9C2 cells

R. V. Devkar, A. V. Pandya and N. H. Shah, Food Funct., 2012, 3, 837 DOI: 10.1039/C2FO00001F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements