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Volume 218, 2019
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Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine

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Abstract

Metabolite identification and annotation procedures are necessary for the discovery of biomarkers indicative of phenotypes or disease states, but these processes can be bottlenecked by the sheer complexity of biofluids containing thousands of different compounds. Here we describe low-cost novel SPE-NMR protocols utilising different cartridges and conditions, on both natural and artificial urine mixtures, which produce unique retention profiles useful for metabolic profiling. We find that different SPE methods applied to biofluids such as urine can be used to selectively retain metabolites based on compound taxonomy or other key functional groups, reducing peak overlap through concentration and fractionation of unknowns and hence promising greater control over the metabolite annotation/identification process.

Graphical abstract: Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine

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Article information


Submitted
02 Dec 2018
Accepted
06 Dec 2018
First published
21 Jan 2019

This article is Open Access

Faraday Discuss., 2019,218, 395-416
Article type
Paper

Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine

D. McGill, E. Chekmeneva, J. C. Lindon, Z. Takats and J. K. Nicholson, Faraday Discuss., 2019, 218, 395
DOI: 10.1039/C8FD00220G

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