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Volume 166, 2013
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Peptide nanofibres as molecular transporters: from self-assembly to in vivo degradation

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Abstract

Peptide nanofibres (PNFs) have gained increasing interest as engineered biomaterials for drug delivery and tissue repair because of the versatility in design they offer through the self-assembly of amphiphilic peptide molecules. Their self-assembly is governed by hydrophobic interactions and hydrogen bonds between peptide sequences able to form β-sheets. In this report, we describe the self-assembly of PNFs by using palmitoyl-peptide molecules containing two different cationic amino acid sequences and offer a description of the nanofiber physicochemical characteristics. The structural degradation of these PNFs in physiologically-relevant media was evaluated experimentally and two mechanisms are proposed. We also piloted the tracking of PNFs intracellularly in vitro, upon interaction with primary neuronal cultures, and intracranially in vivo, after stereotactic administration deep within the brain using two types of fluorescent labelled PNFs. Overall, the self-assembled PNFs were seen to internalise within neurons and be removed or degrade in the brain. Further work is needed to determine the utility of such PNFs as molecular transporters within neuronal tissue.

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Article information


Submitted
17 Jun 2013
Accepted
15 Jul 2013
First published
17 Jul 2013

Faraday Discuss., 2013,166, 181-194
Article type
Paper

Peptide nanofibres as molecular transporters: from self-assembly to in vivo degradation

M. Mazza, A. Patel, R. Pons, C. Bussy and K. Kostarelos, Faraday Discuss., 2013, 166, 181
DOI: 10.1039/C3FD00100H

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