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Volume 161, 2013
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Formation and analysis of topographical domains between lipid membranes tethered by DNA hybrids of different lengths

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Abstract

We recently described a strategy to prepare DNA-tethered lipid membranes either to fixed DNA on a surface or to DNA displayed on a supported bilayer [Boxer et al., J. Struct. Biol., 2009, 168, 190; Boxer et al., Langmuir, 2011, 27, 5492]. With the latter system, the DNA hybrids are laterally mobile; when orthogonal sense–antisense pairs of different lengths are used, the DNA hybrids segregate by height and the tethered membrane deforms to accommodate the height difference. This architecture is particularly useful for modelling interactions between membranes mediated by molecular recognition and resembles cell-to-cell junctions. The length, affinity and population of the DNA hybrids between the membranes are completely controllable. Interesting patterns of height segregation are observed by fluorescence interference contrast microscopy. Diverse behavior is observed in the segregation and pattern forming process and possible mechanisms are discussed. This model system captures some of the essential physics of synapse formation and is a step towards understanding lipid membrane behaviour in cell-to-cell junctions.

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Publication details

The article was received on 15 May 2012, accepted on 11 Jun 2012 and first published on 08 Oct 2012


Article type: Paper
DOI: 10.1039/C2FD20108A
Faraday Discuss., 2013,161, 333-345

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    Formation and analysis of topographical domains between lipid membranes tethered by DNA hybrids of different lengths

    M. Chung, B. J. Koo and S. G. Boxer, Faraday Discuss., 2013, 161, 333
    DOI: 10.1039/C2FD20108A

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