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Volume 149, 2011
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Development of reagents and assays for the detection of pathogenic Burkholderia species

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Abstract

Rapid detection of the category B biothreat agents Burkholderia pseudomallei and Burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for B. pseudomallei). We are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. We present our current medium-throughout approach for target selection and production of Burkholderiamacromolecules and describe the generation of a Fab molecule targeted to the B. malleiBimA protein. We also present development of prototype assays for detecting Burkholderia species using anti-lipopolysaccharide antibodies.

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Publication details

The article was received on 03 Jun 2010, accepted on 19 Jul 2010 and first published on 22 Nov 2010


Article type: Paper
DOI: 10.1039/C005422B
Faraday Discuss., 2011,149, 23-36

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    Development of reagents and assays for the detection of pathogenic Burkholderia species

    O. Qazi, M. Rani, A. J. Gnanam, T. W. Cullen, C. M. Stead, H. Kensing, K. McCaul, S. Ngugi, J. L. Prior, A. Lipka, J. M. Nagy, G. C. Whitlock, B. M. Judy, S. V. Harding, R. W. Titball, S. S. Sidhu, M. S. Trent, G. B. Kitto, A. Torres, D. M. Estes, B. Iverson, G. Georgiou and K. A. Brown, Faraday Discuss., 2011, 149, 23
    DOI: 10.1039/C005422B

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