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Volume 149, 2011
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Combining functionalised nanoparticles and SERS for the detection of DNA relating to disease

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Abstract

DNA functionalised nanoparticle probes offer new opportunities in analyte detection. Ultrasensitive, molecularly specific targeting of analytes is possible through the use of metallic nanoparticles and their ability to generate a surface enhanced Raman scattering (SERS) response. This is leading to a new range of diagnostic clinical probes based on SERS detection. Our approaches have shown how such probes can detect specific DNA sequences by using a biomolecular recognition event to ‘turn on’ a SERS response through a controlled assembly process of the DNA functionalised nanoparticles. Further, we have prepared DNA aptamer functionalised SERS probes and demonstrated how introduction of a protein target can change the aggregation state of the nanoparticles in a dose-dependant manner. These approaches are being used as methods to detect biomolecules that indicate a specific disease being present with a view to improving disease management.

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Article information


Submitted
26 May 2010
Accepted
10 Jun 2010
First published
23 Sep 2010

Faraday Discuss., 2011,149, 291-299
Article type
Paper

Combining functionalised nanoparticles and SERS for the detection of DNA relating to disease

D. Graham, R. Stevenson, D. G. Thompson, L. Barrett, C. Dalton and K. Faulds, Faraday Discuss., 2011, 149, 291
DOI: 10.1039/C005397J

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