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Issue 10, 2011
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Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production

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Abstract

To better understand how hydrothermal pretreatment reduces plant cell wall recalcitrance, we applied a high throughput approach (“glycome profiling”) using a comprehensive suite of plant glycan-directed monoclonal antibodies to monitor structural/extractability changes in Populus biomass. The results of glycome profiling studies were verified by immunolabeling using selected antibodies from the same toolkit. The array of monoclonal antibodies employed in these studies is large enough to monitor changes occurring in most plant cell wall polysaccharides. Results from these techniques demonstrate the sequence of structural changes that occur in plant cell walls during pretreatment-induced deconstruction, namely, the initial disruption of lignin-polysaccharide interactions in concert with a loss of pectins and arabinogalactans; this is followed by significant removal of xylans and xyloglucans. Additionally, this study also suggests that lignin content per se does not affect recalcitrance; instead, the integration of lignin and polysaccharides within cell walls, and their associations with one another, play a larger role.

Graphical abstract: Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production

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Publication details

The article was received on 10 Jul 2011, accepted on 17 Aug 2011 and first published on 06 Sep 2011


Article type: Paper
DOI: 10.1039/C1EE02112E
Citation: Energy Environ. Sci., 2011,4, 4332-4339

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    Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production

    J. D. DeMartini, S. Pattathil, U. Avci, K. Szekalski, K. Mazumder, M. G. Hahn and C. E. Wyman, Energy Environ. Sci., 2011, 4, 4332
    DOI: 10.1039/C1EE02112E

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