Jump to main content
Jump to site search

Issue 43, 2016

Folic acid conjugated Fe3O4 magnetic nanoparticles for targeted delivery of doxorubicin

Author affiliations

Abstract

The interfacial engineering of magnetic nanoparticles (MNPs) with specific functional groups or targeting ligands is important for their in vivo applications. We report here the preparation and characterization of bifunctional magnetic nanoparticles (BMNPs) which contain a carboxylic moiety for drug binding and an amine moiety for folate mediated drug targeting. BMNPs were prepared by introducing bioactive cysteine molecules onto the surface of undecenoic acid coated Fe3O4 magnetic nanoparticles (UMNPs) via a thiol–ene click reaction and then, folic acid was conjugated with these BMNPs through an EDC–NHS coupling reaction. X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis indicate the formation of highly crystalline single-phase Fe3O4 nanostructures. The changes in the interfacial characteristics of the nanoparticles and the presence of an organic coating are evident from Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), zeta-potential measurement, and thermogravimetric analysis (TGA). These nanocarriers have an average size of 10 nm, and have a pH dependent charge conversional feature and protein resistance characteristic in physiological medium. These nanoparticles also show high loading affinity for an anticancer drug, doxorubicin hydrochloride (DOX) and its pH dependent release. This is highly beneficial for cancer therapy as the relatively low pH in tumors will specifically stimulate the drug release at the site of interest. Furthermore, our fluorescence microscopy and flow cytometry studies confirmed the higher cellular internalization capability of these folic acid conjugated nanoparticles in cancer cells over-expressing folate receptors.

Graphical abstract: Folic acid conjugated Fe3O4 magnetic nanoparticles for targeted delivery of doxorubicin

Supplementary files

Article information


Submitted
24 Aug 2016
Accepted
28 Sep 2016
First published
28 Sep 2016

Dalton Trans., 2016,45, 17401-17408
Article type
Paper

Folic acid conjugated Fe3O4 magnetic nanoparticles for targeted delivery of doxorubicin

S. Rana, N. G. Shetake, K. C. Barick, B. N. Pandey, H. G. Salunke and P. A. Hassan, Dalton Trans., 2016, 45, 17401 DOI: 10.1039/C6DT03323G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements