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Issue 14, 2015
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A new selective chromogenic and turn-on fluorogenic probe for copper(II) in solution and vero cells: recognition of sulphide by [CuL]

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Abstract

A new coumarin-appended thioimidazole-linked imine conjugate, viz. L has been synthesized and characterized. L has been found to recognize Cu2+ selectively among a wide range of biologically relevant metal ions. The chemosensing behavior of L has been demonstrated through fluorescence, absorption, visual fluorescence color changes, ESI-MS and 1H NMR titrations. The chemosensor L showed selectivity toward Cu2+ by switch on fluorescence among the 18 metal ions studied with a detection limit of 1.53 μM. The complex formed between L and Cu2+ is found to be 1 : 1 on the basis of absorption and fluorescence titrations and was confirmed by ESI-MS. DFT and TDDFT calculations were performed in order to demonstrate the structure of L and [CuL] and the electronic properties of chemosensor L and its copper complex. This highly fluorescent [CuL] complex has been used to recognize sulphide selectively among the other allied anions. Microstructural features of L and its Cu2+ complex have been investigated by SEM imaging (scanning electron microscopy). The biological applications of L were evaluated in Vero cells and it was found to exhibit low cytotoxicity and good membrane permeability for the detection of Cu2+.

Graphical abstract: A new selective chromogenic and turn-on fluorogenic probe for copper(ii) in solution and vero cells: recognition of sulphide by [CuL]

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Publication details

The article was received on 23 Dec 2014, accepted on 17 Feb 2015 and first published on 17 Feb 2015


Article type: Paper
DOI: 10.1039/C4DT03969F
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Citation: Dalton Trans., 2015,44, 6490-6501

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    A new selective chromogenic and turn-on fluorogenic probe for copper(II) in solution and vero cells: recognition of sulphide by [CuL]

    A. K. Mahapatra, S. Mondal, S. K. Manna, K. Maiti, R. Maji, Md. R. Uddin, S. Mandal, D. Sarkar, T. K. Mondal and D. K. Maiti, Dalton Trans., 2015, 44, 6490
    DOI: 10.1039/C4DT03969F

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