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Issue 8, 2015

Solution studies on DNA interactions of substitution-inert platinum complexes mediated via the phosphate clamp

Author affiliations

Abstract

The phosphate clamp is a distinct mode of ligand–DNA binding where the molecular recognition is manifested through (“non-covalent”) hydrogen-bonding from am(m)ines of polynuclear platinum complexes to the phosphate oxygens on the oligonucleotide backbone. This third mode of DNA binding is unique to the “classical” DNA intercalators and minor groove binding agents and even the closely related covalently binding mononuclear and polynuclear drugs. 2D 1H NMR studies on the Dickerson-Drew dodecamer (DDD, d(CGCGAATTCGCG)2) showed significant A–T contacts mainly on nucleotides A6, T7 and T8 implying a selective bridging from C9G10 in the 3′ direction to C9G10 of the opposite strand. {1H, 15N} HSQC NMR spectroscopy using the fully 15N-labelled compound [{trans-Pt(NH2)3(H2N(CH2)6NH3}2μ-(H2N(CH2)6NH2)2(Pt(NH3)2]8+ (TriplatinNC) showed at pH 6 significant chemical shifts and 1J(195Pt–15N) coupling constants for the free drug and DDD-TriplatinNC at pH 7 indicative of formation of the phosphate clamp. 31P NMR results are also reported for the hexamer d(CGTACG)2 showing changes in 31P NMR chemical shifts indicative of changes around the phosphorus center. The studies confirm the DNA binding modes by substitution-inert (non-covalent) polynuclear platinum complexes and help in further establishing the chemotype as a new class of potential anti-tumour agents in their own right with a distinct profile of biological activity.

Graphical abstract: Solution studies on DNA interactions of substitution-inert platinum complexes mediated via the phosphate clamp

Supplementary files

Article information


Submitted
20 Oct 2014
Accepted
03 Dec 2014
First published
04 Dec 2014

Dalton Trans., 2015,44, 3563-3572
Article type
Paper
Author version available

Solution studies on DNA interactions of substitution-inert platinum complexes mediated via the phosphate clamp

Y. Qu, R. G. Kipping and N. P. Farrell, Dalton Trans., 2015, 44, 3563 DOI: 10.1039/C4DT03237C

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