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Issue 16, 2013
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[Ru(bpy)2dppz-idzo]2+: a colorimetric molecular “light switch” and powerful stabilizer for G-quadruplex DNA

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Abstract

A new ruthenium complex, [Ru(bpy)2dppz-idzo]2+ (bpy = 2,2′-bipyridine, dppz-idzo = dipyrido-[3,2-a:2′,3′-c] phenazine-imidazolone), was synthesized and characterized. The luminescent titrations showed that the Ru-complex exhibited an outstanding “light switch” effect with an emission enhancement factor of about 300 in the presence of G-quadruplex DNA in a K+ solution. This remarkable “light switch” behavior can even be observed by the naked eye under irradiation with UV light. To get an insight into the “light switch” mechanism, quantum-chemical calculations were performed based on the DFT/TDDFT/PCM method at the B3LYP/6-31G* level. Furthermore, the CD titrations and thermal melting experiments indicated that [Ru(bpy)2dppz-idzo]2+ could not only induce the formation of an antiparallel G-quadruplex structure in the absence of monocations, but also has the ability to stabilize the G-quadruplex architecture, implying potential applications in anticancer therapeutics. Both the “light switch” effect and the structure stabilization ability of [Ru(bpy)2dppz-idzo]2+ were found to be superior to the well-known DNA molecular “light switch” [Ru(bpy)2dppz]2+. Finally, a “sandwich-like” binding model was proposed on the basis of molecular docking simulations.

Graphical abstract: [Ru(bpy)2dppz-idzo]2+: a colorimetric molecular “light switch” and powerful stabilizer for G-quadruplex DNA

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Publication details

The article was received on 05 Nov 2012, accepted on 22 Jan 2013 and first published on 23 Jan 2013


Article type: Paper
DOI: 10.1039/C3DT32640C
Citation: Dalton Trans., 2013,42, 5661-5672

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    [Ru(bpy)2dppz-idzo]2+: a colorimetric molecular “light switch” and powerful stabilizer for G-quadruplex DNA

    J. Yao, X. Gao, W. Sun, S. Shi and T. Yao, Dalton Trans., 2013, 42, 5661
    DOI: 10.1039/C3DT32640C

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