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Issue 36, 2010
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Synthesis of hybrid transition-metalloproteinsviathiol-selective covalent anchoring of Rh-phosphine and Ru-phenanthroline complexes

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Abstract

The preparation of hybrid transition metalloproteins by thiol-selective incorporation of organometallic rhodium- and ruthenium complexes is described. Phosphine ligands and two rhodium-diphosphine complexes bearing a carboxylic acid group were coupled to the cysteine of PYP R52G, yielding a metalloenzyme active in the rhodium catalyzed hydrogenation of dimethyl itaconate. The successful coupling was shown by 31P NMR spectroscopy and ESI mass spectroscopy. In addition wild-type PYP (PYP WT), PYP R52G and ALBP were successfully modified with a (η6-arene) ruthenium(II) phenanthroline complex via a maleimide linker.

Graphical abstract: Synthesis of hybrid transition-metalloproteins via thiol-selective covalent anchoring of Rh-phosphine and Ru-phenanthroline complexes

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Publication details

The article was received on 31 Mar 2010, accepted on 10 Jun 2010 and first published on 06 Jul 2010


Article type: Paper
DOI: 10.1039/C0DT00239A
Dalton Trans., 2010,39, 8477-8483

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    Synthesis of hybrid transition-metalloproteins via thiol-selective covalent anchoring of Rh-phosphine and Ru-phenanthroline complexes

    R. den Heeten, B. K. Muñoz, G. Popa, W. Laan and P. C. J. Kamer, Dalton Trans., 2010, 39, 8477
    DOI: 10.1039/C0DT00239A

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