Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 6, 1997
Previous Article Next Article

Structural model of rabbit liver coppermetallothionein

Abstract

Molecular modelling techniques have been used to calculate the structure for the primary, mammalian copper-containing protein, metallothionein Cu 12 -MT. Structural information obtained from existing spectroscopic data and comparison with inorganic copper(I)–thiolate model compounds were employed. A two-domain structure, with stoichiometries of Cu 6 S 9 and Cu 6 S 11 , was constructed in which the copper(I)–cysteine connections were based on the arrangement of the cysteines in the Zn 7 -MT precursor as determined by previous 1 H NMR studies. In the minimized structure, in which metal–thiolate bond lengths and angles were allowed to vary, each copper(I) was trigonally co-ordinated by cysteine thiolates; the average bond length was calculated to be close to 224 pm. In the α domain of the protein both bridging (7S) and terminal (4S) thiolate ligation take part in the Cu 6 S 11 cluster structure, in the β domain all nine thiolates bridge pairs of Cu I atoms to form the Cu 6 S 9 cluster structure. Significantly, the energy-minimized structure exhibits two main crevices that are similar to those reported for the structure of Cd 5 Zn 2 -MT 1 obtained by analysis of X-ray diffraction data. These crevices could allow access to the otherwise embedded copper–thiolate clusters.

Back to tab navigation

Article type: Paper
DOI: 10.1039/A605462E
Citation: J. Chem. Soc., Dalton Trans., 1997,0, 977-984

  •   Request permissions

    Structural model of rabbit liver copper metallothionein

    A. Presta, D. A. Fowle and M. J. Stillman, J. Chem. Soc., Dalton Trans., 1997, 0, 977
    DOI: 10.1039/A605462E

Search articles by author

Spotlight

Advertisements