Issue 12, 1991

Spectroscopic studies of nickel(II) carbonic anhydrase and its adducts with inorganic anions

Abstract

Nickel(II) carbonic anhydrase, NiBCA II, and its adducts with nitrate, acetate, cyanate and azide, have been investigated through 1H NMR and electronic absorption spectroscopies. From the pH dependence of the molar absorbance the acidity constants of NiBCA were determined. The anions bind to the metal ion forming 1 : 1 adducts, and the corresponding affinity constants have been determined. The 1H NMR spectra of NiBCA and its adducts have been recorded, and the signals corresponding to the meta-like protons of the co-ordinated histidines followed by 1H NMR titration. The T1 values of these signals were measured and resonance assignments made based on nuclear Overhauser enhancement experiments. The co-ordination geometry of the metal ion in NiBCA and its adducts is discussed on the basis of the temperature dependence of the isotropic shifts, molar absorbance, and longitudinal relaxation times.

Article information

Article type
Paper

J. Chem. Soc., Dalton Trans., 1991, 3393-3399

Spectroscopic studies of nickel(II) carbonic anhydrase and its adducts with inorganic anions

J. M. Moratal, M. Martínez-Ferrer, A. Donaire, J. Castells, J. Salgado and H. R. Jiménez, J. Chem. Soc., Dalton Trans., 1991, 3393 DOI: 10.1039/DT9910003393

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements