Issue 5, 2016

A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards α,ω-dinitriles

Abstract

The β37 residue of nitrile hydratase (NHase) from Pseudomonas putida and NHase from Comamonas testosteroni played a critical role in directing enzyme regioselectivity. Amino acid substitution in this site modulated or even inverted enzyme regioselectivity towards aliphatic α,ω-dinitriles.

Graphical abstract: A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards α,ω-dinitriles

Supplementary files

Article information

Article type
Communication
Submitted
20 Nov 2015
Accepted
15 Jan 2016
First published
18 Jan 2016

Catal. Sci. Technol., 2016,6, 1292-1296

A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards α,ω-dinitriles

Z. Cheng, W. Cui, Z. Liu, L. Zhou, M. Wang, M. Kobayashi and Z. Zhou, Catal. Sci. Technol., 2016, 6, 1292 DOI: 10.1039/C5CY01997D

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