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Issue 8, 2012
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Porous cross linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase

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Abstract

In this work, porous cross-linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase are reported for the first time. Porous CLEAs were prepared by adding starch as a pore-making agent in crude invertase solution and co-precipitating with invertase using ammonium sulfate as a precipitating agent. The resultant co-precipitate was cross-linked for 8 h with 20 mM glutaraldehyde and in the end starch was removed by alpha amylase. The highest catalytic efficiency was obtained at 0.4% (w/v) starch. The scanning electron microscopy analysis of invertase p-CLEAs showed a porous structure. The p-CLEAs were shown to have improved internal mass transfer compared to conventional CLEAs.

Graphical abstract: Porous cross linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase

  • This article is part of the themed collection: Biocatalysis
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Publication details

The article was received on 08 May 2012, accepted on 08 Jun 2012 and first published on 13 Jun 2012


Article type: Paper
DOI: 10.1039/C2CY20304A
Catal. Sci. Technol., 2012,2, 1575-1579

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    Porous cross linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase

    S. Talekar, V. Shah, S. Patil and M. Nimbalkar, Catal. Sci. Technol., 2012, 2, 1575
    DOI: 10.1039/C2CY20304A

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