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Issue 15, 2013
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Immobilisation and application of lipases in organic media

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Different methods of preparing lipases for use in organic media are critically reviewed. Solid lipase preparations can be made by typical immobilisation methods such as adsorption, entrapment, covalent coupling or cross-linking. Immobilisation is especially attractive for lipases because, in addition to the normal benefits of enzyme immobilisation, it can also lead to a considerable increase in catalytic activity, probably caused by conformational changes in the lipase molecules. Activation can be achieved, for example, using hydrophobic support materials or surfactants during the immobilisation procedure. Surfactants can also be used to solubilise lipases in organic media via the formation of hydrophobic ion pairs, surfactant-coated lipase or reversed micelles. Lipase preparation methods are discussed with regard to potential lipase inactivation and activation effects, mass transfer limitations, lipase stability and other features important for applications. The practical applications of lipases in organic media reviewed include ester synthesis, modification of triacylglycerols and phospholipids, fatty acid enrichment, enantiomer resolution, biodiesel production and acylation of carbohydrates and bioactive compounds.

Graphical abstract: Immobilisation and application of lipases in organic media

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Publication details

The article was received on 29 Oct 2012 and first published on 12 Feb 2013

Article type: Review Article
DOI: 10.1039/C3CS35446F
Chem. Soc. Rev., 2013,42, 6406-6436
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    Immobilisation and application of lipases in organic media

    P. Adlercreutz, Chem. Soc. Rev., 2013, 42, 6406
    DOI: 10.1039/C3CS35446F

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