Jump to main content
Jump to site search

Issue 12, 2011
Previous Article Next Article

Artificial DNA cutters for DNA manipulation and genome engineering

Author affiliations

Abstract

This tutorial review provides recent developments in artificial cutters for site-selective scission of DNA with the focus on chemistry-based DNA cutters. They are useful tools for molecular biology and biotechnology, since their site-selectivity of scission is much higher than that of naturally occurring restriction enzymes and also their scission site is freely chosen. In order to prepare these cutters, a DNA-cutting molecule is combined with a sequence-recognizing molecule in a covalent or non-covalent way. At targeted sites in single-stranded and double-stranded DNAs, the scission occurs via either oxidative cleavage of nucleotides or hydrolysis of phosphodiester linkages. Among many successful examples, an artificial restriction DNA cutter, prepared from Ce(IV)/EDTA and pseudo-complementary peptide nucleic acid, hydrolyzed double-stranded DNA at the target site. The scission site and scission specificity are determined simply in terms of the Watson–Crick rule so that even the whole genome of human beings was selectively cut at one predetermined site. Consistently, homologous recombination in human cells was successfully promoted by this tool. For the purpose of comparison, protein-based DNA cutters (e.g., zinc finger nucleases) are also briefly described. The potential applications of these cutters and their future aspects are discussed.

Graphical abstract: Artificial DNA cutters for DNA manipulation and genome engineering

Back to tab navigation

Publication details

The article was received on 09 Feb 2011 and first published on 12 May 2011


Article type: Tutorial Review
DOI: 10.1039/C1CS15039A
Chem. Soc. Rev., 2011,40, 5657-5668

  •   Request permissions

    Artificial DNA cutters for DNA manipulation and genome engineering

    Y. Aiba, J. Sumaoka and M. Komiyama, Chem. Soc. Rev., 2011, 40, 5657
    DOI: 10.1039/C1CS15039A

Search articles by author

Spotlight

Advertisements