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Issue 41, 2020
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Revealing unfolding steps and volume changes of human telomeric i-motif DNA

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Abstract

Cytosine rich DNA sequences can fold into a non-canonical four stranded intercalated i-motif structure. We investigated Htel-iM, an i-motif structure of the human telomeric DNA region that plays an important role in cell division, cancer diseases and aging. A high pressure up to 1 Gpa was applied to reveal the volumetric changes during unfolding. Thermal transitions at different pressures were followed by infrared and fluorescence spectroscopy. We demonstrated that Htel-iM unfolds in two steps. First the outer hydrogen bonds break in which C(2)[double bond, length as m-dash]O(2) and the N(4)H2 amino group is involved, subsequently the hydrogen bond involving N(3)+ becomes broken. Htel-iM was destabilized by pressure and unfolded with a negative volume change.

Graphical abstract: Revealing unfolding steps and volume changes of human telomeric i-motif DNA

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Article information


Submitted
22 Jul 2020
Accepted
28 Sep 2020
First published
01 Oct 2020

Phys. Chem. Chem. Phys., 2020,22, 23816-23823
Article type
Paper

Revealing unfolding steps and volume changes of human telomeric i-motif DNA

J. Somkuti, O. R. Molnár and L. Smeller, Phys. Chem. Chem. Phys., 2020, 22, 23816
DOI: 10.1039/D0CP03894F

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