Issue 41, 2016

Osmotic pressures of lysozyme solutions from gas-like to crystal states

Abstract

We obtained osmotic pressure data of lysozyme solutions, describing their physical states over a wide concentration range, using osmotic stress for pressures between 0.05 bar and about 40 bar and volume fractions between 0.01 and 0.61. The osmotic pressure vs. volume fraction data consist of a dilute, gas-phase regime, a transition regime with a high-compressibility plateau, and a concentrated regime where the system is nearly incompressible. The first two regimes are shifted towards a higher protein volume fraction upon decreasing the strength or the range of electrostatic interactions. We describe this shift and the overall shape of the experimental data in these two regimes through a model accounting for a steric repulsion, a short-range van der Waals attraction and a screened electrostatic repulsion. The transition is caused by crystallization, as shown by small-angle X-ray scattering. We verified that our data points correspond to thermodynamic equilibria, and thus that they consist of the reference experimental counterpart of a thermodynamic equation of state.

Graphical abstract: Osmotic pressures of lysozyme solutions from gas-like to crystal states

Supplementary files

Article information

Article type
Paper
Submitted
03 Jun 2016
Accepted
16 Sep 2016
First published
16 Sep 2016

Phys. Chem. Chem. Phys., 2016,18, 28458-28465

Osmotic pressures of lysozyme solutions from gas-like to crystal states

C. Pasquier, S. Beaufils, A. Bouchoux, S. Rigault, B. Cabane, M. Lund, V. Lechevalier, C. Le Floch-Fouéré, M. Pasco, G. Pabœuf, J. Pérez and S. Pezennec, Phys. Chem. Chem. Phys., 2016, 18, 28458 DOI: 10.1039/C6CP03867K

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