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Issue 31, 2014
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Accessing the third dimension in localization-based super-resolution microscopy

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Abstract

Only a few years after its inception, localization-based super-resolution microscopy has become widely employed in biological studies. Yet, it is primarily used in two-dimensional imaging and accessing the organization of cellular structures at the nanoscale in three dimensions (3D) still poses important challenges. Here, we review optical and computational techniques that enable the 3D localization of individual emitters and the reconstruction of 3D super-resolution images. These techniques are grouped into three main categories: PSF engineering, multiple plane imaging and interferometric approaches. We provide an overview of their technical implementation as well as commentary on their applicability. Finally, we discuss future trends in 3D localization-based super-resolution microscopy.

Graphical abstract: Accessing the third dimension in localization-based super-resolution microscopy

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Article information


Submitted
30 Mar 2014
Accepted
16 May 2014
First published
05 Jun 2014

Phys. Chem. Chem. Phys., 2014,16, 16340-16348
Article type
Perspective
Author version available

Accessing the third dimension in localization-based super-resolution microscopy

B. Hajj, M. El Beheiry, I. Izeddin, X. Darzacq and M. Dahan, Phys. Chem. Chem. Phys., 2014, 16, 16340
DOI: 10.1039/C4CP01380H

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