An intriguing pH-triggered FRET-based biosensor emission of a pyrazoline–doxorubicin couple and its application in living cells†
Abstract
A unique pH-driven Förster resonance energy transfer (FRET) based biosensor emission by a pyrazoline–doxorubicin pair has been deciphered with a bioimaging application in a live HepG2 cell whereas conformational switching of both molecules at elevated pH reveals a fascinating twist (FRET-OFF) via strong fluorescent exciplex formation.