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Issue 43, 2012
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Vacuum-ultraviolet circular dichroism reveals DNA duplex formation between short strands of adenine and thymine

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Abstract

Absorbance spectroscopy is used extensively to tell when two DNA single strands come together and form a double strand. Here we show that circular dichroism in the vacuum ultraviolet region provides an even stronger indication for duplex formation in the case of short strands of adenine and thymine (4 to 16 bases in each strand). Indeed, our results show that a strong positive CD band appears at 179 nm when double strands are formed. Melting experiments were done in aqueous solution with and without added Na+ counter ions. With additional salt present a huge increase in the 179 nm CD band was observed when lowering the temperature. A 179 nm CD marker band for duplex formation can be used to measure the kinetics for the association of two single strands. Such experiments rely on large changes at one particular wavelength since it is too time-consuming to record a full-wavelength spectrum.

Graphical abstract: Vacuum-ultraviolet circular dichroism reveals DNA duplex formation between short strands of adenine and thymine

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Article information


Submitted
09 Dec 2011
Accepted
07 Aug 2012
First published
08 Aug 2012

Phys. Chem. Chem. Phys., 2012,14, 15054-15059
Article type
Paper

Vacuum-ultraviolet circular dichroism reveals DNA duplex formation between short strands of adenine and thymine

L. M. Nielsen, S. V. Hoffmann and S. Brøndsted Nielsen, Phys. Chem. Chem. Phys., 2012, 14, 15054
DOI: 10.1039/C2CP42226C

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