Jump to main content
Jump to site search

Issue 17, 2010
Previous Article Next Article

Quantification of the adhesion strength of fibroblastcells on ethylene glycol terminated self-assembled monolayers by a microfluidic shear force assay

Author affiliations

Abstract

The adhesion strength of cells depends on the properties of the surface they attach to. Varying the surface properties can trigger different cellular responses such as differentiation. In order to study cell adhesion quantitatively, we developed a microfluidic shear force assay which allows the variation of applied shear stress by five orders of magnitude. With this device we can determine the critical shear stress which is necessary to remove 50% of the adherent cells. As an application we investigated the adhesion strength of cells on a series of oligo(ethylene glycol) (OEG) containing self-assembled monolayers (SAMs). By varying the number of ethylene oxide units, the hydration properties of the monolayers are changed. We found that cell adhesion strength for mammalian fibroblasts decreases if the hydration of the surface is increased. As the cell spreading area changes with the substrate properties, the adhesion strength per unit area was additionally determined.

Graphical abstract: Quantification of the adhesion strength of fibroblast cells on ethylene glycol terminated self-assembled monolayers by a microfluidic shear force assay

Back to tab navigation

Article information


Submitted
18 Nov 2009
Accepted
20 Jan 2010
First published
03 Mar 2010

Phys. Chem. Chem. Phys., 2010,12, 4498-4504
Article type
Paper

Quantification of the adhesion strength of fibroblast cells on ethylene glycol terminated self-assembled monolayers by a microfluidic shear force assay

C. Christophis, M. Grunze and A. Rosenhahn, Phys. Chem. Chem. Phys., 2010, 12, 4498
DOI: 10.1039/B924304F

Social activity

Search articles by author

Spotlight

Advertisements