Fabrication of 2D-protein arrays using biotinylated thiols: results from fluorescence microscopy and atomic force microscopy

Abstract

The coupling of a fluorescently labelled protein, streptavidin, to biotinylated organic surfaces is investigated using fluorescence microscopy (FM) and atomic force microscopy (AFM). In order to study the importance of non-specific adsorption investigations were carried out using two-dimensionally structured self-assembled monolayers (SAMs), which were fabricated using micro-contact printing. The relative amount of specific vs. non-specific adsorption could be readily determined by comparing the amount of streptavidin adsorbed on adjacent regions consisting of biotinylated organothiolates and of protein-resistant oligoethyleneglycol (OEG)-thiols.