Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.

Issue 8, 2000
Previous Article Next Article

On the role of methylene blue in the oscillating peroxidase–oxidase reaction

Author affiliations


The role of methylene blue (MB) and its interaction with various enzymic and nonenzymic intermediates in the oscillating peroxidase–oxidase reaction were investigated. Time dependent spectral changes were deconvoluted into time series of the different intermediates. Ferric peroxidase, ferrous peroxidase, and peroxidase compound III account for most of the spectral changes, while the two remaining oxidation states of the enzyme (compound I and II) are only present in very low amounts during any phase of the oscillations. The presence of MB is required for the generation of sustained oscillations and complex dynamics. The amount of ferrous peroxidase formed during an oscillatory cycle decreases with increasing concentrations of MB. At ‘‘high ’’ concentrations of MB only damped oscillations are observed. These findings are interpreted in terms of either a competition between MB and other reactants for NAD radicals, or of a reaction between MB and ferrous peroxidase. In the absence of MB only damped oscillations can be observed due to slow and irreversible degradation of the enzyme. This suggests that MB also protects the enzyme against degradation.

Back to tab navigation

Article information

23 Dec 1999
15 Feb 2000
First published
05 Jan 2000

Phys. Chem. Chem. Phys., 2000,2, 1685-1692
Article type

On the role of methylene blue in the oscillating peroxidase–oxidase reaction

M. J. B. Hauser, A. Lunding and L. F. Olsen, Phys. Chem. Chem. Phys., 2000, 2, 1685
DOI: 10.1039/A910319H

Social activity

Search articles by author