Issue 14, 2018

Application of protein crystallization methodologies to enhance the solubility, stability and monodispersity of proteins

Abstract

Protein crystallization screens are commonly used to find specific crystallization conditions. In practice, undesirable clear drops are often ignored. We hypothesize that clear drops may be potentially useful for finding the conditions leading to proteins with high solubility and monodispersity . Based on this idea, we first tested three model proteins and confirmed that improved monodispersity could be observed in the clear drops. Next, we used this strategy to achieve the maximum solubility and monodispersity of a new protein (RNA binding motif 3, RBM3). We suggest that this strategy is useful not only for high-throughput screening of optimal solubilization conditions but also for screening of biopharmaceutical formulations.

Graphical abstract: Application of protein crystallization methodologies to enhance the solubility, stability and monodispersity of proteins

Supplementary files

Article information

Article type
Communication
Submitted
19 Dec 2017
Accepted
24 Feb 2018
First published
24 Feb 2018

CrystEngComm, 2018,20, 1923-1927

Application of protein crystallization methodologies to enhance the solubility, stability and monodispersity of proteins

R. Zhou, X. Lu, C. Dong, F. Ahmad, C. Zhang and D. Yin, CrystEngComm, 2018, 20, 1923 DOI: 10.1039/C7CE02189E

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