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Issue 46, 2021

Sensitive, general and portable detection of RNAs combining duplex-specific nuclease transduction with an off-shelf signalling platform

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Abstract

We report a novel RNA sensing platform by combining the RNA:DNA hybridization, duplex-specific nuclease (DSN) amplification, and personal glucose meter (PGM) readout. The so-called DSN–PGM sensing platform is sensitive, specific and general to both microRNA and long virus RNA. The detection procedure is simple and instrument-free, thus holding particular potential in the development of portable and point-of-care measurements.

Graphical abstract: Sensitive, general and portable detection of RNAs combining duplex-specific nuclease transduction with an off-shelf signalling platform

Supplementary files

Article information


Submitted
31 Mar 2021
Accepted
30 Apr 2021
First published
30 Apr 2021

Chem. Commun., 2021,57, 5714-5717
Article type
Communication

Sensitive, general and portable detection of RNAs combining duplex-specific nuclease transduction with an off-shelf signalling platform

L. Guo, H. Li, R. Zhao, Y. Tang and B. Li, Chem. Commun., 2021, 57, 5714 DOI: 10.1039/D1CC01715B

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