Issue 51, 2021

Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2

Abstract

Tracking the viral progression of SARS-CoV-2 in COVID-19 infected body tissues is an emerging need of the current pandemic. Imaging at near infrared second biological window (NIR-II) offers striking benefits over the other technologies to explore deep-tissue information. Here we design, synthesise and characterise a molecular probe that selectively targets the N-gene of SARS-CoV-2. Highly specific antisense oligonucleotides (ASOs) were conjugated to lead sulfide quantum dots using a UV-triggered thiol–ene click chemistry for the recognition of viral RNA. Our ex vivo imaging studies demonstrated that the probe exhibits aggregation induced NIR-II emission only in presence of SARS-CoV-2 RNA which can be attributed to the efficient hybridisation of the ASOs with their target RNA strands.

Graphical abstract: Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2

Supplementary files

Article information

Article type
Communication
Submitted
18 Mar 2021
Accepted
24 May 2021
First published
24 May 2021

Chem. Commun., 2021,57, 6229-6232

Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2

P. Moitra, M. Alafeef, K. Dighe, Z. Sheffield, D. Dahal and D. Pan, Chem. Commun., 2021, 57, 6229 DOI: 10.1039/D1CC01410B

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