Primer design and condition optimization for PCR are tedious and labour-intensive. To conveniently achieve high selectivity, sensitivity and robustness, herein, we first report a new strategy with Se-dNTPs to enhance PCR specificity (over 240-fold) and sensitivity (up to single-digit), effectively eliminating non-specific products and simplifing PCR design and optimization.
B. Hu, Y. Wang, N. Li, S. Zhang, G. Luo and Z. Huang, Chem. Commun., 2021, 57, 57 DOI: 10.1039/D0CC06172G
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