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Issue 83, 2020
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CRISPR-Cas12a trans-cleaves DNA G-quadruplexes

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Abstract

We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.

Graphical abstract: CRISPR-Cas12a trans-cleaves DNA G-quadruplexes

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Article information


Submitted
14 Aug 2020
Accepted
13 Sep 2020
First published
23 Sep 2020

Chem. Commun., 2020,56, 12526-12529
Article type
Communication

CRISPR-Cas12a trans-cleaves DNA G-quadruplexes

Y. Li, T. Li, B. Liu, R. Hu, J. Zhu, T. He, X. Zhou, C. Li, Y. Yang and M. Liu, Chem. Commun., 2020, 56, 12526 DOI: 10.1039/D0CC05540A

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