Issue 85, 2020

Precise engineering of apoferritin through site-specific host–guest binding

Abstract

The surface engineering of the apoferritin shell by means of traditional chemical modifications usually suffers from site inaccuracy and insufficient conjugation. This report describes a non-covalent method for precise modulation of the apoferritin surface without alteration of amino acid residues. A bifunctional macromolecule, structured as azide–poly(ethylene glycol)–porphyrin (termed TPA), was synthesized. TPA was observed to be able to recognize and bind apoferritin in a 12 : 1 stoichiometry with a higher binding affinity than arachidonate, thanks to the specific host–guest interaction between the pocket of each two-fold channel and the porphyrin moiety. This method allows for site-specific engineering of the apoferritin surface with on demand functionalities and optimization of drug encapsulation.

Graphical abstract: Precise engineering of apoferritin through site-specific host–guest binding

Supplementary files

Article information

Article type
Communication
Submitted
07 Aug 2020
Accepted
21 Sep 2020
First published
21 Sep 2020

Chem. Commun., 2020,56, 12897-12900

Precise engineering of apoferritin through site-specific host–guest binding

X. Wang, K. Du, H. Heng, W. Chen, X. Li, X. Wei, F. Feng and S. Wang, Chem. Commun., 2020, 56, 12897 DOI: 10.1039/D0CC05382A

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