Design of spontaneously blinking fluorophores for live-cell super-resolution imaging based on quantum-chemical calculations

Ryo Tachibana; Mako Kamiya; Akihiko Morozumi; Yoshiyuki Miyazaki; Hiroyoshi Fujioka; Aika Nanjo; Ryosuke Kojima; Toru Komatsu; Tasuku Ueno; Kenjiro Hanaoka; Toshitada Yoshihara; Seiji Tobita; Yasuteru Urano

doi:10.1039/D0CC05126H

Design of spontaneously blinking fluorophores for live-cell super-resolution imaging based on quantum-chemical calculations†

Ryo Tachibana,^a Mako Kamiya,

*^b Akihiko Morozumi,

^a Yoshiyuki Miyazaki,^a Hiroyoshi Fujioka,

^a Aika Nanjo,^a Ryosuke Kojima,

^bc Toru Komatsu,

^a Tasuku Ueno,

^a Kenjiro Hanaoka,

^a Toshitada Yoshihara,

^d Seiji Tobita

^d and Yasuteru Urano*^abe

Author affiliations

* Corresponding authors

^a Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: uranokun@m.u-tokyo.ac.jp

^b Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: mkamiya@m.u-tokyo.ac.jp

^c PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

^d Graduate School of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu-shi, Gunma 376-8515, Japan

^e AMED-CREST, Japan Agency for Medical Research and Development, 1-7-1 Otemachi, Chiyoda-ku, Tokyo 100-0004, Japan

Abstract

Spontaneously blinking fluorophores are powerful tools for live-cell super-resolution imaging under physiological conditions. Here we show that quantum-chemical calculations can predict key parameters for fluorophore design. We applied this methodology to develop a spontaneously blinking fluorophore with yellow fluorescence for super-resolution imaging of microtubules in living cells.

Chemical Communications

Design of spontaneously blinking fluorophores for live-cell super-resolution imaging based on quantum-chemical calculations†

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Design of spontaneously blinking fluorophores for live-cell super-resolution imaging based on quantum-chemical calculations

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