Krypton-derivatization highlights O2-channeling in a four-electron reducing oxidase

Sylvain Engilberge; Tristan Wagner; Philippe Carpentier; Eric Girard; Seigo Shima

doi:10.1039/D0CC04557H

Krypton-derivatization highlights O₂-channeling in a four-electron reducing oxidase†

Sylvain Engilberge,

‡^a Tristan Wagner,

‡^b Philippe Carpentier,

^cd Eric Girard

*^e and Seigo Shima

*^f

Author affiliations

* Corresponding authors

^a Paul Scherrer Institut, Forschungsstrasse 111, 5232 Villigen PSI, Switzerland

^b Microbial Metabolism Group, Max-Planck-Institut für Marine Mikrobiologie, Celsiusstraße 1, Bremen, Germany

^c Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Laboratoire Chimie et Biologie des Métaux (LCBM), Université Grenoble Alpes, CNRS, CEA, Grenoble, France

^d European Synchrotron Radiation Facility (ESRF), Grenoble, France

^e Univ. Grenoble Alpes, CEA, CNRS, IBS, F-38000 Grenoble, France
E-mail: eric.girard@ibs.fr

^f Microbial Protein Structure Group, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Straße 10, 35043 Marburg, Germany
E-mail: shima@mpi-marburg.mpg.de

Abstract

F₄₂₀H₂-oxidase (FprA) catalyses the four-electron reduction of O₂ to 2H₂O using the reduced form of F₄₂₀ as electron donor. The hydrophobic O₂-channel detected by Kr-derivatization and the concerted movement of a gating loop could contribute to prevent unwanted side-reaction between the catalytic intermediates and solvents, therefore preventing reactive oxygen species formation.

This article is part of the themed collection: (Photo)electrocatalysis for renewable energy

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Article information

DOI: https://doi.org/10.1039/D0CC04557H
Article type: Communication
Submitted: 01 Jul 2020
Accepted: 03 Aug 2020
First published: 03 Aug 2020
This article is Open Access

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Chem. Commun., 2020,56, 10863-10866

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Krypton-derivatization highlights O₂-channeling in a four-electron reducing oxidase

S. Engilberge, T. Wagner, P. Carpentier, E. Girard and S. Shima, Chem. Commun., 2020, 56, 10863 DOI: 10.1039/D0CC04557H

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Chemical Communications