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Issue 77, 2020

Functionalization of colloidal nanoparticles with a discrete number of ligands based on a “HALO-bioclick” reaction

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Abstract

A recombinant HALO-GFP fusion protein was designed and isolated to demonstrate the feasibility of controlling the number and orientation of protein ligands to be conjugated on colloidal gold nanoparticles. AuNPs functionalized with exactly one or exactly two GFP molecules exhibited fully preserved functionality of the protein. The method is very straightforward and generally provides highly bioactive nanoparticle–protein conjugates.

Graphical abstract: Functionalization of colloidal nanoparticles with a discrete number of ligands based on a “HALO-bioclick” reaction

Supplementary files

Article information


Submitted
23 Jun 2020
Accepted
21 Aug 2020
First published
21 Aug 2020

Chem. Commun., 2020,56, 11398-11401
Article type
Communication

Functionalization of colloidal nanoparticles with a discrete number of ligands based on a “HALO-bioclick” reaction

S. Garbujo, E. Galbiati, L. Salvioni, M. Mazzucchelli, G. Frascotti, X. Sun, S. Megahed, N. Feliu, D. Prosperi, W. J. Parak and M. Colombo, Chem. Commun., 2020, 56, 11398 DOI: 10.1039/D0CC04355A

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