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Issue 84, 2020
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A novel, rationally designed lanthanoid chelating tag delivers large paramagnetic structural restraints for biomolecular NMR

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Abstract

Herein, a novel and rationally designed ortho-substituted pyridine activator is reported that reacts rapidly and selectively with cysteine thiols. It forms reduction-stable conjugates and induces large pseudocontact shifts, residual dipolar couplings and paramagnetic relaxation enhancement on both ubiquitin S57C and human carbonic anhydrase II S50C constructs under physiological conditions.

Graphical abstract: A novel, rationally designed lanthanoid chelating tag delivers large paramagnetic structural restraints for biomolecular NMR

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Supplementary files

Article information


Submitted
22 Jun 2020
Accepted
10 Sep 2020
First published
14 Sep 2020

This article is Open Access

Chem. Commun., 2020,56, 12861-12864
Article type
Communication

A novel, rationally designed lanthanoid chelating tag delivers large paramagnetic structural restraints for biomolecular NMR

D. Joss, F. Winter and D. Häussinger, Chem. Commun., 2020, 56, 12861
DOI: 10.1039/D0CC04337K

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