Issue 62, 2020
From the journal:

Chemical Communications

An in vitro site-specific cleavage assay of CRISPR-Cas9 using a personal glucose meter†

Shaohua Gong, ORCID logo a   Yuanyuan Chen, ORCID logo a   Wei Pan, ORCID logo *a   Na Li ORCID logo *a  and  Bo Tang ORCID logo *a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China
E-mail: panwei@sdnu.edu.cn, lina@sdnu.edu.cn, tangb@sdnu.edu.cn

Abstract

A sensitive and cost-effective assay based on a personal glucose meter was developed to detect the in vitro site-specific cleavage efficiency of CRISPR-Cas9.

Graphical abstract: An in vitro site-specific cleavage assay of CRISPR-Cas9 using a personal glucose meter

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Article information

DOI
https://doi.org/10.1039/D0CC03505J
Article type
Communication
Submitted
16 May 2020
Accepted
29 Jun 2020
First published
30 Jun 2020

Chem. Commun., 2020,56, 8850-8853

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An in vitro site-specific cleavage assay of CRISPR-Cas9 using a personal glucose meter

S. Gong, Y. Chen, W. Pan, N. Li and B. Tang, Chem. Commun., 2020, 56, 8850 DOI: 10.1039/D0CC03505J

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