Cell-free protein synthesis in hydrogel materials†
Colette J.
Whitfield, 
a
Alice M.
Banks,
a
Gema
Dura,
a
John
Love,
b
Jonathan E.
Fieldsend,
c
David A.
Fulton
a
and
Thomas P.
Howard
*a
a
Alice M.
Banks,
a
Gema
Dura,
a
John
Love,
b
Jonathan E.
Fieldsend,
c
David A.
Fulton
a
and
Thomas P.
Howard
*a
Abstract
We report a method for embedding cell-free protein synthesis reactions in macro-scale hydrogel materials without a free liquid phase. This paper focuses on methods of preparation for a variety of hydrogels and an investigation of the impact that the hydrogel material has on cell-free protein synthesis.
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