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Issue 55, 2020
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The engineering of decameric d-fructose-6-phosphate aldolase A by combinatorial modulation of inter- and intra-subunit interactions

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Abstract

The combinatorial modulation of inter- and intra-subunit interactions of decameric D-fructose-6-phosphate aldolase A (FSAA) generated a triple-site variant I31T/Q59T/I195Q FSAA with 27- to 278-fold improvement in activity towards target heteroaromatic aldehydes. X-ray crystallographic data and molecular dynamics simulations ascribed the enhanced activity to the pronounced flexibility of the interface region between subunits, the expanded substrate entrance and binding pocket, and enhanced proton transfer, unambiguously demonstrating the efficiency of this strategy for engineering multimeric enzymes.

Graphical abstract: The engineering of decameric d-fructose-6-phosphate aldolase A by combinatorial modulation of inter- and intra-subunit interactions

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Supplementary files

Article information


Submitted
04 Apr 2020
Accepted
01 May 2020
First published
07 May 2020

Chem. Commun., 2020,56, 7561-7564
Article type
Communication

The engineering of decameric D-fructose-6-phosphate aldolase A by combinatorial modulation of inter- and intra-subunit interactions

X. Yang, L. Wu, A. Li, L. Ye, J. Zhou and H. Yu, Chem. Commun., 2020, 56, 7561
DOI: 10.1039/D0CC02437F

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