Stiffness of cationized gelatin nanoparticles is a key factor determining RNAi efficiency in myeloid leukemia cells

Weiqi Zhang; Bo Han; Xinning Lai; Chen Xiao; Shilin Xu; Xianghui Meng; Zifu Li; Jie Meng; Tao Wen; Xiangliang Yang; Jian Liu; Haiyan Xu

doi:10.1039/C9CC09068A

Stiffness of cationized gelatin nanoparticles is a key factor determining RNAi efficiency in myeloid leukemia cells†

Weiqi Zhang,

‡^ab Bo Han,‡^a Xinning Lai,^a Chen Xiao,^c Shilin Xu,^a Xianghui Meng,^a Zifu Li,

^cde Jie Meng,^a Tao Wen,^a Xiangliang Yang,^cde Jian Liu*^a and Haiyan Xu

*^a

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* Corresponding authors

^a Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, P. R. China
E-mail: xuhy@pumc.edu.cn, liujian@ibms.pumc.edu.cn

^b State Key Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, P. R. China

^c Department of Nanomedicine and Biopharmaceuticals, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China

^d National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan, China

^e Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Huazhong University of Science and Technology, Wuhan, China

Abstract

Here we demonstrated that the stiffness of cationized gelatin nanoparticles determined the efficiency of RNAi in myeloid leukemia cells when the particle size and surface charges were kept constant. The siRNA delivery system with an elastic modulus of 0.87 MPa showed the largest siRNA uptake and RNAi efficiency for hard-to-transfect suspension cells.

Chemical Communications

Stiffness of cationized gelatin nanoparticles is a key factor determining RNAi efficiency in myeloid leukemia cells†

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Stiffness of cationized gelatin nanoparticles is a key factor determining RNAi efficiency in myeloid leukemia cells

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