Issue 87, 2019

Selective and sensitive fluorescence “turn-on” detection of 4-thiouridine in nucleic acids via oxidative amination

Abstract

A fluorescence “turn-on” method for digestion-free analysis of 4-thiouridine (s4U) in nucleic acids was developed in this work based on the oxidative amination of s4U by fluoresceinamine (FAM-NH2) and periodate (IO4). It was 125-fold more sensitive for s4U detection than the traditional UV330 absorption method, and showed excellent selectivity to s4U over 2-thiouridine (s2U) analogues and biological thiols.

Graphical abstract: Selective and sensitive fluorescence “turn-on” detection of 4-thiouridine in nucleic acids via oxidative amination

Supplementary files

Article information

Article type
Communication
Submitted
14 Aug 2019
Accepted
07 Oct 2019
First published
09 Oct 2019

Chem. Commun., 2019,55, 13096-13099

Selective and sensitive fluorescence “turn-on” detection of 4-thiouridine in nucleic acids via oxidative amination

J. Wang, J. Shang, Z. Qin, A. Tong and Y. Xiang, Chem. Commun., 2019, 55, 13096 DOI: 10.1039/C9CC06312A

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