Issue 80, 2019
From the journal:

Chemical Communications

Rapid and room temperature detection of single nucleotide variation with enhanced discrimination by crowding assisted allele specific extension†

Miao Cui,‡ab   Xiaoyu Zhou,‡a   Xiaoyu Chen,‡c   Weihao Zheng,d   Liming Bian,c   Zigang Li ORCID logo d  and  Bo Zheng ORCID logo *a  

* Corresponding authors

a Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
E-mail: bozheng@cuhk.edu.hk

b Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China

c Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong, China

d School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, China

Abstract

In this paper we report the kinetics based detection of single nucleotide variation (SNV) at room temperature by allele specific extension with different concentrations and types of crowding agents. In general, the crowding conditions enhanced the specificity in the detection of SNV.

Graphical abstract: Rapid and room temperature detection of single nucleotide variation with enhanced discrimination by crowding assisted allele specific extension

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Article information

DOI
https://doi.org/10.1039/C9CC06229G
Article type
Communication
Submitted
11 Aug 2019
Accepted
10 Sep 2019
First published
10 Sep 2019

Chem. Commun., 2019,55, 12052-12055

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Rapid and room temperature detection of single nucleotide variation with enhanced discrimination by crowding assisted allele specific extension

M. Cui, X. Zhou, X. Chen, W. Zheng, L. Bian, Z. Li and B. Zheng, Chem. Commun., 2019, 55, 12052 DOI: 10.1039/C9CC06229G

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