A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamers

Lele Sheng; Yunhao Lu; Sha Deng; Xinyu Liao; Kaixiang Zhang; Tian Ding; Hong Gao; Donghong Liu; Ruijie Deng; Jinghong Li

doi:10.1039/C9CC05036A

A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamers†

Lele Sheng,^ab Yunhao Lu,^a Sha Deng,^a Xinyu Liao,^b Kaixiang Zhang,^cd Tian Ding,*^b Hong Gao,^a Donghong Liu,

^b Ruijie Deng

*^a and Jinghong Li

^c

Author affiliations

* Corresponding authors

^a College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China
E-mail: drj17@scu.edu.cn

^b Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, Zhejiang, China
E-mail: tding@zju.edu.cn

^c Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China

^d School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China

Abstract

We report a transcription aptasensor by using a light-up RNA aptamer. It allows for sensitive, label-free and culture-free detection of intact foodborne pathogens, and no separation, purification or enrichment processes are involved.

Chemical Communications

A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamers†

Abstract

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A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamers

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