Issue 48, 2019
From the journal:

Chemical Communications

Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification†

Yan Zhang,‡a   Xin-yan Wang,‡a   Xianwei Su‡b  and  Chun-yang Zhang ORCID logo *a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn
Fax: +86 531-82615258
Tel: +86 531-86186033

b CUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China

Abstract

We develop a new fluorescent method for sensitive detection of long noncoding RNAs (lncRNAs) on the basis of duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification. This method exhibits high sensitivity with a detection limit of 0.081 fM, and it can accurately detect the endogenous lncRNA HOTAIR in cancer cells, providing a new approach to study the physiological function of lncRNAs in human diseases.

Graphical abstract: Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification

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Article information

DOI
https://doi.org/10.1039/C9CC02939G
Article type
Communication
Submitted
16 Apr 2019
Accepted
13 May 2019
First published
13 May 2019

Chem. Commun., 2019,55, 6827-6830

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Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification

Y. Zhang, X. Wang, X. Su and C. Zhang, Chem. Commun., 2019, 55, 6827 DOI: 10.1039/C9CC02939G

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