In situ fluorescent profiling of living cell membrane proteins at a single-molecule level

Abstract

We propose a signal amplification method that enables visualization analysis of membrane proteins on living cells at a single-molecule level. Using the proposed method, we achieved imaging of PTK7 membrane proteins on HeLa cells and analyzed the down-regulated expression of EpCAM on the MCF-7 cell surface during epithelial–mesenchymal transition (EMT).